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BP701T

INSTRUMENTAL METHODS OF ANALYSIS

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Unit I: UV Visible Spectroscopy And Fluorimetry

Syllabus Topics to Learn

UV Visible spectroscopy: Electronic transitions
Chromophores
Auxochromes
Spectral shifts
Solvent effect on absorption spectra
Beer and Lambert’s law
Derivation and deviations. Instrumentation - Sources of radiation
Wavelength selectors
Sample cells
Detectors-Photo tube
Photomultiplier tube
Photo voltaic cell
Silicon Photodiode. Applications- Spectrophotometric titrations
Single component and multi component analysis. Fluorimetry: Theory
Concepts of singlet
Doublet and triplet electronic states
Internal and external conversions
Factors affecting fluorescence
Quenching
Instrumentation and applications.

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Subject Overview

Course Code BP701T
Syllabus Status 100% PCI Aligned
Total Units 5 Units
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